Requirement for AP-2α in cardiac outflow tract morphogenesis
نویسندگان
چکیده
Most developing structures that express the transcription factor gene AP-2a are compromised in AP-2a mutant mouse embryos. Since the cardiac neural crest population is one prominent site of AP-2a expression, and because the neural crest is known to be required for normal cardiac morphogenesis, we have investigated the involvement of AP-2a in cardiac development. All AP-2a-deficient embryos examined had malformations of the outflow tract of the developing heart: most had double outlet right ventricle, and a small fraction had persistent truncus arteriosus. To visualize AP-2a-expressing cells during the period of cardiac morphogenesis, we established a new mutant germline allele in which an IRES-lacZ sequence was inserted by homologous recombination into the AP-2a locus. Positive expression was observed in the cardiac neural crest population during the E9.5–10.5 period (as well as in other known domains of AP-2a expression previously noted by in situ hybridization studies), and was mostly extinguished by E11.5 when the cardiac neural crest has migrated into the outflow tract of the developing heart. Importantly, the distribution of AP-2a-expressing cardiac neural crest appeared to be identical in normal and mutant embryos. From this analysis, we propose that the AP-2a gene functions within the neural crest lineage, that AP-2a is not required for neural crest cell migration, and that normal AP-2a gene function is required prior to E11.5. AP-2a may be involved in an interaction between neural crest and surrounding tissues in the subpharyngeal region, thereby promoting normal outflow tract morphogenesis. q 2002 Elsevier Science Ireland Ltd. All rights reserved.
منابع مشابه
miRNA-processing enzyme Dicer is necessary for cardiac outflow tract alignment and chamber septation.
MicroRNAs (miRNAs) have previously been implicated in a number of developmental processes, including development of the ventricular myocardium of the heart. To determine what, if any, additional roles miRNAs play in cardiogenesis, we deleted the miRNA-processing enzyme Dicer specifically in the developing murine heart. Embryos lacking cardiac Dicer lived longer than reported in previous studies...
متن کاملCellular components and signals required for the cardiac outflow tract assembly in Drosophila.
Specification of cardiac primordia and formation of the Drosophila heart tube is highly reminiscent of the early steps of vertebrate heart development. We previously reported that the final morphogenesis of the Drosophila heart involves a group of nonmesodermal cells called heart-anchoring cells and a pair of derived from the pharyngeal mesoderm cardiac outflow muscles. Like the vertebrate card...
متن کاملComputational hemodynamic optimization predicts dominant aortic arch selection is driven by embryonic outflow tract orientation in the chick embryo.
In the early embryo, a series of symmetric, paired vessels, the aortic arches, surround the foregut and distribute cardiac output to the growing embryo and fetus. During embryonic development, the arch vessels undergo large-scale asymmetric morphogenesis to form species-specific adult great vessel patterns. These transformations occur within a dynamic biomechanical environment, which can play a...
متن کاملFoxp1 regulates cardiac outflow tract, endocardial cushion morphogenesis and myocyte proliferation and maturation.
We have recently described a new subfamily of Fox genes, Foxp1/2/4, which are transcriptional repressors and are thought to regulate important aspects of development in several tissues, including the lung, brain, thymus and heart. Here, we show that Foxp1 is expressed in the myocardium as well as the endocardium of the developing heart. To further explore the role of Foxp1 in cardiac developmen...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Mechanisms of Development
دوره 110 شماره
صفحات -
تاریخ انتشار 2002